TOMM70 ELISA Kit (Human) (OKCD01085)

Catalog No: OKCD01085 (Formerly GWB-KBBCX2)

Size:96WELLS

Human TOMM70 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TOMM70 Microplate: 96 Wells (12 x 8 Well Strips)
  • TOMM70 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated TOMM70 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A). No significant cross-reactivity or interference between Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TOMM70
• Gene Full Name: translocase of outer mitochondrial membrane 70
• Alias Symbols: mitochondrial import receptor subunit TOM70;mitochondrial precursor proteins import receptor;Tom70;TOMM70A;translocase of outer membrane 70 kDa subunit;translocase of outer mitochondrial membrane 70 homolog A;translocase of outer mitochondrial membrane protein 70.
• NCBI Gene Id: 9868
• Protein Name: Mitochondrial import receptor subunit TOM70
• Description of Target: Receptor that accelerates the import of all mitochondrial precursor proteins.
• Uniprot ID: O94826
• Protein Accession #: NP_055635
• Nucleotide Accession #: NM_014820.4
• Protein Size (# AA): 608