TNS3 ELISA Kit (Human) (OKCD00479)

Catalog No: OKCD00479 (Formerly GWB-KBBWL6)

Size:96WELLS

Human TNS3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	97-105%	81-94%	80-96%	90-103%
  EDTA plasma(n=5)	87-94%	78-89%	83-97%	78-89%
  heparin plasma(n=5)	95-102%	86-94%	93-101%	84-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tensin 3 (TNS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tensin 3 (TNS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tensin 3 (TNS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tensin 3 (TNS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	79-103	84
  EDTA plasma(n=5)	96-105	102
  heparin plasma(n=5)	97-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tensin 3 (TNS3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tensin 3 (TNS3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TNS3 Microplate: 96 Wells (12 x 8 Well Strips)
  • TNS3 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated TNS3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.052 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tensin 3 (TNS3). No significant cross-reactivity or interference between Tensin 3 (TNS3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TNS3
• Gene Full Name: tensin 3
• Alias Symbols: TEM6;TENS1;tensin-3;tensin-like SH2 domain containing 1;tensin-like SH2 domain-containing protein 1;thyroid specific PTB domain protein;tumor endothelial marker 6.
• NCBI Gene Id: 64759
• Protein Name: Tensin-3
• Description of Target: May play a role in actin remodeling. Involved in the dissociation of the integrin-tensin-actin complex. EGF activates TNS4 and down-regulates TNS3 which results in capping the tail of ITGB1. Seems to be involved in mammary cell migration. May be involved in cell migration and bone development (By similarity).
• Uniprot ID: Q68CZ2
• Protein Accession #: NP_073585
• Nucleotide Accession #: NM_022748.11
• Protein Size (# AA): 1445