TNNT1 Antibody (OAAD00208)

Catalog No: OAAD00208

Size:100 ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Rabbit, Chicken
• Clonality: Monoclonal
• Clone: TT-98
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC, IP
• Reconstitution and Storage: At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
• Immunogen: Troponin T from rabbit skeletal muscle.
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human, mouse, rat, rabbit, chicken.
  No cross reactivity with other proteins.
• Formulation: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
• Application Info: Western blot At 2-4ug/ml with the appropriate system to detect Troponin T in cells and tissues.
  Immunohistochemistry(P) At 4-8ug/ml to detect Troponin T in formalin fixed and paraffin embedded tissues.
  Immunohistochemistry(F) At 4-8ug/ml to detect Troponin T in formalin or acetone fixed tissues.
  Other applications have not been tested.
  Optimal dilutions should be determined by end user.
• Storage Buffer: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Reference: 1. Samson, F.; de Jong, P. J.; Trask, B. J.; Koza-Taylor, P.; Speer, M. C.; Potter, T.; Roses, A. D.; Gilbert, J. R. : Assignment of the human slow skeletal troponin T gene to 19q13.4 using somatic cell hybrids and fluorescence in situ hybridization analysis. Genomics 13: 1374-1375, 1992.
  2. Durand, J.-B.; Bachinski, L. L.; Bieling, L. C.; Czernuszewicz, G. Z.; Abchee, A. B.; Yu, Q. T.; Tapscott, T.; Hill, R.; Ifegwu, J.; Marian, A. J.; Brugada, R.; Daiger, S.; Gregoritch, J. M.; Anderson, J.
  L.; Quinones, M.; Towbin, J. A.; Roberts, R. : Localization of a gene responsible for familial dilated cardiomyopathy to chromosome 1q32. Circulation 92: 3387-3389, 1995.
  3. Mao, C.; Baumgartner, A. P.; Jha, P. K.; Huang, T. H.-M.; Sarkar, S. : Assignment of the human fast skeletal troponin T gene (TNNT3) to chromosome 11p15.5: evidence for the presence of 11pter in a monochromosome 9 somatic cell hybrid in NIGMS mapping panel 2. Genomics 31: 385-388, 1996.

Target Info

• Gene Symbol: TNNT1
• Gene Full Name: Troponin T, slow skeletal muscle
• Alias Symbols: ANM, TNT, NEM5, STNT, TNTS
• NCBI Gene Id: 7138
• Protein Name: Troponin T, slow skeletal muscle
• Description of Target: The troponin complex is located on the thin filament of striated muscle and is composed of 3 component polypeptides: troponin 1, troponin T and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in slow skeletal muscle (TNNT1), cardiac muscle(TNNT2), and fast skeletal muscle (TNNT3). TNNT1, TNNT2, TNNT3 are located on 19q13.4, 1q32, 11p15.5 respectively.
• Uniprot ID: P13805
• Protein Accession #: NP_001119604.1; NP_001119605.1; NP_001278703.1; NP_003274.3
• Nucleotide Accession #: NM_001126132.2; NM_001126133.2; NM_001291774.1; NM_003283.5