TNNI3 ELISA Kit (Rabbit) (OKCA02208)

Catalog No: OKCA02208

Size:96 Wells

Rabbit TNNI3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rabbit
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	87	81-92
  Serum (n=4)	1:2	96	92-99
  Serum (n=4)	1:4	94	89-98
  Serum (n=4)	1:8	92	88-96

• ELISA Kit Principle: Aviva Systems Biology TNNI3 ELISA Kit (Rabbit) (OKCA02208) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNNI3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNNI3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TNNI3 captured in well.
• ELISA Kit Range: 47 pg/mL-3,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	87	83-94
  EDTA plasma (n=4)	89	85-97

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-TNNI3 Microplate: 96 Wells (12 x 8 Well strips)
  • TNNI3 Lyophilized Standard: 2 x 3 ng
  • 100X Biotinylated TNNI3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 11.75 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TNNI3
• Protein Name: Troponin I, cardiac muscle
• Description of Target: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
• Uniprot ID: P02646