TNNC1 Antibody (OABB00731)

Catalog No: OABB00731

Size:100UG

WB:Rat Skeletal Muscle Tissue Lysate

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Troponin C1(TNNC1)(troponin C type 1(slow)), also called TROPONIN C, SLOW-TWITCH SKELETAL MUSCLE or TNC, is a protein that in humans is encoded by the TNNC1 gene. Using a "monochromosomal" hybrid panel, the TNNC1 gene is mapped to chromosome 3; subsequent analysis of the Genebridge 4 radiation hybrid panel localized the gene to 3p in a region consistent with the earlier assignment. Circular dichroism measurements revealed changes in the secondary structures of the TNNC1 mutants A8V, C84Y, and D145E.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Rat: Human, Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
• Reference: 1. Bermingham, N., Hernandez, D., Balfour, A., Gilmour, F., Martin, J. E., Fisher, E. M.
  C. Mapping TNNC1, the gene that encodes cardiac troponin I in the human and mouse. Genomics
  30: 620-622, 1995.
  2. Hoffmann, B., Schmidt-Traub, H., Perrot, A., Osterziel, K. J., Gessner, R. First mutation in cardiac
  troponin C, L29Q, in a patient with hypertrophic cardiomyopathy. Hum. Mutat. 17: 524 only, 2001.
  3. Landstrom, A. P., Parvatiyar, M. S., Pinto, J. R., Marquardt, M. L., Bos, J. M., Tester, D. J., Ommen,
  S. R., Potter, J. D., Ackerman, M. J.Molecular and functional characterization of novel hypertrophic
  cardiomyopathy susceptibility mutations in TNNC1-encoded troponin C. J. Molec. Cell. Cardiol. 45:
  281-288, 2008.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Troponin C, slow skeletal and cardiac muscles(TNNC1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: TNNC1
• Gene Full Name: troponin C1, slow skeletal and cardiac type
• Alias Symbols: cardiac troponin C;CMD1Z;CMH13;slow twitch skeletal/cardiac muscle troponin C;TNC;TN-C;TNNC;troponin C type 1 (slow);troponin C, slow skeletal and cardiac muscles.
• NCBI Gene Id: 7134
• Protein Name: Troponin C, slow skeletal and cardiac muscles
• Description of Target: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
• Uniprot ID: P63316
• Protein Accession #: NP_003271.1
• Nucleotide Accession #: NM_003280.2
• Molecular Weight: 18403 MW