TNMD ELISA Kit (Rat) (OKEH04398)

Catalog No: OKEH04398

Size:96T

Rat TNMD ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Tnmd ELISA Kit (Rat) (OKEH04398) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Tnmd antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tnmd and incubated. The found in the sample or standards competes with the biotinylated Tnmd for limited binding sites on the immobilized anti-Rat Tnmd antibody. Excess unbound biotinylated Tnmd and sample or standard Tnmd is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tnmd captured in the well and inversely proportional to the amount of Tnmd which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Component:
  • Anti-Tnmd Microplate: 96 Wells (12 x 8 Well Strips)
  • Tnmd Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Tnmd Biotinylated Complex: 1 x 60 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.089 ng/mL
• Specificity: Natural and recombinant Rat Tenomodulin
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Tnmd
• Gene Full Name: tenomodulin
• Alias Symbols: ChM1L;chondromodulin-1-like protein;chondromodulin-I-like protein;myodulin;rChM1L;rTeM;teM;tenomodulin.
• NCBI Gene Id: 64104
• Protein Name: Q9ESC2
• Description of Target: Tenomodulin
• Uniprot ID: https://www.uniprot.org/uniprot/Q9ESC2
• Protein Accession #: NP_071626.1
• Nucleotide Accession #: NM_022290.1
• Protein Size (# AA): 317
• Molecular Weight: 37 KDa