TNKS Antibody - N-terminal region (OAAB11199)

Catalog No: OAAB11199

Size:400ul

TNKS Antibody (N-term) (Cat. #OAAB11199) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TNKS antibody detected the TNKS protein (arrow).

TNKS Antibody (N-term) (OAAB11199)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TNKS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: TNKS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1
  Cellular Location: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Chromosome, centromere. Nucleus, nuclear pore complex. Chromosome, telomere (Potential). Cytoplasm, cytoskeleton, spindle pole. Note=Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During int
  Tissue Location: Ubiquitous; highest levels in testis.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This TNKS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-82 amino acids from the N-terminal region of human TNKS.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: TNKS
• Alias Symbols: TIN1, ARTD5, PARPL, TINF1, TNKS1, pART5, PARP5A, PARP-5a
• NCBI Gene Id: 8658
• Protein Name: Tankyrase-1
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O95271
• Protein Accession #: NP_003738.2
• Molecular Weight: 142039 Da