TNK2 Antibody (Phospho-Tyr284) (OAAB20039)

Catalog No: OAAB20039

Size:100ul

Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using ACK1 (Phospho-Tyr284) Antibody (OAAB20039).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) Antibody (OAAB20039).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: WB, IHC, IF
• Additional Information: Function: Non-receptor tyrosine-protein and serine/threonine- protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr- 287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Target Post-Translational Modification: Phospho-Tyr284
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:1000
  IHC: 1:50~100
  IF: 1:100
• Reference: Manser E.,et al.Nature 363:364-367(1993).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Muzny D.M.,et al.Nature 440:1194-1198(2006).
  Eisenmann K.M.,et al.Nat. Cell Biol. 1:507-513(1999).
  Kato J.,et al.Biochem. Biophys. Res. Commun. 268:141-147(2000).
  

Target Info

• Gene Symbol: TNK2
• Gene Full Name: tyrosine kinase, non-receptor, 2
• Alias Symbols: ACK, ACK1, ACK-1, p21cdc42Hs
• NCBI Gene Id: 10188
• Description of Target: This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
• Uniprot ID: Q07912
• Protein Accession #: NP_001010938.1
• Nucleotide Accession #: NM_001010938.1
• Molecular Weight: 115 kDa