TNFSF9 ELISA Kit (Human) (OKCD01793)

Catalog No: OKCD01793 (Formerly GWB-KBBMN1)

Size:96WELLS

Human TNFSF9 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	95-102%	84-103%	80-94%	98-105%
  EDTA Plasma (n=5)	86-94%	80-96%	87-103%	93-101%
  Heparin Plasma (n=5)	95-102%	97-105%	87-94%	95-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	85-95	90
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma (n=5)	87-96	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TNFSF9 Microplate: 96 Wells (12 x 8 Well Strips)
  • TNFSF9 Lyophilized Standard: 2 x 500 pg
  • 100X Biotinylated TNFSF9 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 2.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9). No significant cross-reactivity or interference between Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TNFSF9
• Gene Full Name: TNF superfamily member 9
• Alias Symbols: 4-1BB ligand;4-1BBL;4-1BB-L;CD137 ligand;CD137L;homolog of mouse 4-1BB-L;receptor 4-1BB ligand;TNLG5A;tumor necrosis factor (ligand) superfamily, member 9;tumor necrosis factor ligand 5A;tumor necrosis factor ligand superfamily member 9;tumor necrosis factor superfamily member 9.
• NCBI Gene Id: 8744
• Protein Name: Tumor necrosis factor ligand superfamily member 9
• Description of Target: Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
• Uniprot ID: P41273
• Protein Accession #: NP_003802.1
• Nucleotide Accession #: NM_003811.3
• Protein Size (# AA): 254