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Catalog No: OKEH05964
Size:96T
Price: $625.00
SKU
OKEH05964
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Tnfsf4 ELISA Kit (Rat) (OKEH05964) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tnfsf4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tnfsf4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfsf4 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 95%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <5.4%, n = 20
Mean Inter-Assay Precision - <7.9%, n = 20
ELISA Kit Component
ComponentAmount
TNFSF4 Microplate96 Wells (12 x 8 Well strips)
TNFSF4 Lyophilized Standard2
100X Biotinylated TNFSF4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity40.5 pg/mL
SpecificityNatural and recombinant Rat Tumor necrosis factor ligand superfamily member 4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTnfsf4
Gene Full NameTNF superfamily member 4
Alias SymbolsOX40 ligand;Ox40l;Tnlg2b;tumor necrosis factor (ligand) superfamily, member 4;tumor necrosis factor ligand 2b;tumor necrosis factor ligand superfamily member 4;tumor necrosis factor superfamily member 4.
NCBI Gene Id89814
Protein NameQ9Z2P3
Description of TargetTumor necrosis factor ligand superfamily member 4
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Z2P3
Protein Accession #NP_446004.1
Nucleotide Accession #NM_053552.1
Protein Size (# AA)199
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