Catalog No: OCOA01791
Size:20UG
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Datasheets/Manuals | Printable datasheet for TNFSF13B Protein Lysate (OCOA01791) |
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Predicted Species Reactivity | Homo sapiens |
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Product Format | Lyophilized |
Host | HEK293T |
Additional Information | Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
:: | Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reconstitution and Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes |
Tag | C-MYC/DDK |
Description | TNFSF13B HEK293T cell transient overexpression lysate (as WB positive control) |
Gene Symbol | TNFSF13B |
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Gene Full Name | TNF superfamily member 13b |
Alias Symbols | ApoL related ligand TALL-1;B lymphocyte stimulator;BAFF;B-cell-activating factor;B-lymphocyte stimulator;BLYS;CD257;delta BAFF;Delta4 BAFF;dendritic cell-derived TNF-like molecule;DTL;epididymis secretory sperm binding protein;TALL1;TALL-1;THANK;TNF and ApoL-related leukocyte expressed ligand 1;TNF- and APOL-related leukocyte expressed ligand 1;TNF homolog that activates apoptosis;TNFSF20;TNLG7A;tumor necrosis factor (ligand) superfamily, member 13b;tumor necrosis factor (ligand) superfamily, member 20;tumor necrosis factor ligand 7A;tumor necrosis factor ligand superfamily member 13B;tumor necrosis factor superfamily member 13b;tumor necrosis factor-like protein ZTNF4;ZTNF4. |
NCBI Gene Id | 10673 |
Protein Name | A0A0U5J7Q1, NM_006573, NP_006564, Q9Y275 |
Description of Target | Epididymis secretory sperm binding protein, Homo sapiens TNF superfamily member 13b (TNFSF13B), transcript variant 1, mRNA, Tumor necrosis factor ligand superfamily member 13B, tumor necrosis factor ligand superfamily member 13B isoform 1 [Homo sapiens] |
Uniprot ID | https://www.uniprot.org/uniprot/Q9Y275 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_006564 |
Nucleotide Accession # | https://www.ncbi.nlm.nih.gov/nuccore/NM_006573 |
Molecular Weight | 31.2 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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