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Catalog No: OCOA01791
Size:20UG
Price: $179.00
SKU
OCOA01791
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TNFSF13B Protein Lysate (OCOA01791)

Datasheets/ManualsPrintable datasheet for TNFSF13B Protein Lysate (OCOA01791)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionTNFSF13B HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolTNFSF13B
Gene Full NameTNF superfamily member 13b
Alias SymbolsApoL related ligand TALL-1;B lymphocyte stimulator;BAFF;B-cell-activating factor;B-lymphocyte stimulator;BLYS;CD257;delta BAFF;Delta4 BAFF;dendritic cell-derived TNF-like molecule;DTL;epididymis secretory sperm binding protein;TALL1;TALL-1;THANK;TNF and ApoL-related leukocyte expressed ligand 1;TNF- and APOL-related leukocyte expressed ligand 1;TNF homolog that activates apoptosis;TNFSF20;TNLG7A;tumor necrosis factor (ligand) superfamily, member 13b;tumor necrosis factor (ligand) superfamily, member 20;tumor necrosis factor ligand 7A;tumor necrosis factor ligand superfamily member 13B;tumor necrosis factor superfamily member 13b;tumor necrosis factor-like protein ZTNF4;ZTNF4.
NCBI Gene Id10673
Protein NameA0A0U5J7Q1, NM_006573, NP_006564, Q9Y275
Description of TargetEpididymis secretory sperm binding protein, Homo sapiens TNF superfamily member 13b (TNFSF13B), transcript variant 1, mRNA, Tumor necrosis factor ligand superfamily member 13B, tumor necrosis factor ligand superfamily member 13B isoform 1 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Y275
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_006564
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_006573
Molecular Weight31.2 kDa
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