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Catalog No: OKEH02941
Size:96T
Price: $425.00
SKU
OKEH02941
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TNFSF13B ELISA Kit (Human) : 96 Wells (OKEH02941)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TNFSF13B ELISA Kit (Human) (OKEH02941) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFSF13B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFSF13B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFSF13B captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 10.7% (n = 20)
ELISA Kit Component
ComponentAmount
TNFSF13B Microplate96 Wells (12 x 8 Well strips)
TNFSF13B Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated TNFSF13B Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.2 ng/mL
SpecificityNatural and recombinant Human Tumor necrosis factor ligand superfamily member 13B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTNFSF13B
Gene Full NameTNF superfamily member 13b
Alias SymbolsApoL related ligand TALL-1;B lymphocyte stimulator;BAFF;B-cell-activating factor;B-lymphocyte stimulator;BLYS;CD257;delta BAFF;Delta4 BAFF;dendritic cell-derived TNF-like molecule;DTL;epididymis secretory sperm binding protein;TALL1;TALL-1;THANK;TNF and ApoL-related leukocyte expressed ligand 1;TNF- and APOL-related leukocyte expressed ligand 1;TNF homolog that activates apoptosis;TNFSF20;TNLG7A;tumor necrosis factor (ligand) superfamily, member 13b;tumor necrosis factor (ligand) superfamily, member 20;tumor necrosis factor ligand 7A;tumor necrosis factor ligand superfamily member 13B;tumor necrosis factor superfamily member 13b;tumor necrosis factor-like protein ZTNF4;ZTNF4.
NCBI Gene Id10673
Protein NameQ9Y275
Description of TargetTumor necrosis factor ligand superfamily member 13B
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Y275
Protein Accession #NP_001139117.1
Nucleotide Accession #NM_001145645.2
Protein Size (# AA)285
Molecular Weight31 kDa
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