TNFSF13 ELISA Kit (Human) (OKCD07634)

Catalog No: OKCD07634

Size:96WELLS

TNFSF13 ELISA Kit (Human) (OKCD07634) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 46.88-3,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • TNFSF13 Microplate: 96 Wells (12 x 8 Well strips)
  • TNFSF13 Lyophilized Standard: 2
  • 100X TNFSF13 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 16.53pg/mL

Target Info

• Gene Symbol: TNFSF13
• Gene Full Name: TNF superfamily member 13
• Alias Symbols: a proliferation-inducing ligand;APRIL;CD256;TALL2;TALL-2;TNF- and APOL-related leukocyte expressed ligand 2;TNF-related death ligand 1;TNLG7B;TRDL-1;tumor necrosis factor (ligand) superfamily, member 13;tumor necrosis factor ligand 7B;tumor necrosis factor ligand superfamily member 13;tumor necrosis factor superfamily member 13;tumor necrosis factor-like protein ZTNF2;tumor necrosis factor-related death ligand-1;UNQ383/PRO715;ZTNF2.
• NCBI Gene Id: 8741
• Protein Name: Tumor necrosis factor ligand superfamily member 13
• Description of Target: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
• Uniprot ID: O75888
• Protein Accession #: NP_001185552.1
• Nucleotide Accession #: NM_001198623.1