TNFSF12 Protein (OPPA02220)

Catalog No: OPPA02220 (Formerly GWB-AD8F0E)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl pH-7.5.
• Host: HEK293 (Human Embryonic Kidney Cell Line)
• Application: WB
• Additional Information: Solubility: Add pyrogen free water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile
  Product Introduction: TNFSF12 is a cytokine that is part of the TNF ligand family. TNFSF12 is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TNFSF12 induces apoptosis through multiple pathways of cell death in a cell type-specific manner. TNFSF12 promotes proliferation and migration of endothelial cells, and therefore acts as a regulator of angiogenesis. TNFSF12 is expressed in adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK h induces NFkB and chemokine secretion. TNFSF12 exerts an apoptotic activity in HT-29 human adenocarcinoma cells whilst cultured in the presence of IFN-*. TNFSF12 promotes proliferation and migration of endothelial cells.
  Product Description: The TWEAK Human Recombinant is manufactured with N-terminal fusion of 13 amino acid FLAG Tag. The TNFSF12 Flag -Tagged Fusion Protein is an 18.7kDa protein containing 169 amino acid residues and 13 additional amino acid residues - Flag Tag (underlined).
• Reconstitution and Storage: Store lyophilized TWEAK Human recombinant at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C. The lyophilized protein remains stable until the expiry date when stored at -20C.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Peptide Sequence: HVDYKDDDDK PAGSAPKGRK TRARRAIAAH YEVHPRPGQD GAQAGVDGTV SGWEEARINS SSPLRYNRQI GEFIVTRAGL YYLYCQVHFD EGKAVYLKLD LLVDGVLALR CLEEFSATAA SSLGPQLRLC QVSGLLALRP GSSLRIRTLP WAHLKAAPFL TYFGLFQVH.

Target Info

• Gene Symbol: TNFSF12
• Alias Symbols: APO3L, DR3LG, TWEAK, TNLG4A
• NCBI Gene Id: 8742
• Protein Name: Tumor necrosis factor ligand superfamily member 12
• Description of Target: Recombinant Human TNF Ligand Superfamily Member 12
• Uniprot ID: O43508
• Protein Accession #: NP_003800.1
• Protein Size (# AA): Recombinant