TNFSF10 ELISA Kit (Dog) (OKCD00219)

Catalog No: OKCD00219 (Formerly GWB-KBBYI9)

Size:96WELLS

Dog TNFSF10 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Canis lupus|Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TNFSF10 Microplate: 96 Wells (12 x 8 Well Strips)
  • TNFSF10 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated TNFSF10 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). No significant cross-reactivity or interference between Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TNFSF10
• Gene Full Name: TNF superfamily member 10
• Alias Symbols: TNF-related apoptosis inducing ligand;TNLG6A;TRAIL;tumor necrosis factor (ligand) superfamily, member 10;tumor necrosis factor ligand 6A;tumor necrosis factor ligand superfamily member 10;tumor necrosis factor superfamily member 10.
• NCBI Gene Id: 100174780
• Protein Name: Tumor necrosis factor ligand superfamily member
• Description of Target: Tumor necrosis factor ligand superfamily member
• Uniprot ID: F1PWB1
• Protein Accession #: NP_001124308
• Nucleotide Accession #: NM_001130836.1
• Protein Size (# AA): 281