TNFSF10 Chemi-Luminescent ELISA Kit (Human) (OKCD03998)

Catalog No: OKCD03998

Size:96WELLS

Human TNFSF10 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	99-105%	78-104%	84-94%	90-101%
  EDTA Plasma (n=5)	91-105%	96-104%	98-105%	93-103%
  Heparin Plasma (n=5)	97-105%	96-103%	89-103%	86-103%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) level in the sample or standard.
• ELISA Kit Range: 41.2-30,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93-101	96
  EDTA Plasma (n=5)	98-105	101
  Heparin Plasma (n=5)	93-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • TNFSF10 Microplate: 96 Wells (12 x 8 Well strips)
  • TNFSF10 Lyophilized Standard: 2
  • 100X TNFSF10 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.0412 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TNFSF10
• Gene Full Name: TNF superfamily member 10
• Alias Symbols: Apo-2 ligand;Apo-2L;APO2L;CD253;chemokine tumor necrosis factor ligand superfamily member 10;TL2;TNF-related apoptosis inducing ligand TRAIL;TNF-related apoptosis-inducing ligand;TNLG6A;TRAIL;tumor necrosis factor (ligand) family, member 10;tumor necrosis factor (ligand) superfamily, member 10;tumor necrosis factor apoptosis-inducing ligand splice variant delta;tumor necrosis factor ligand 6A;tumor necrosis factor ligand superfamily member 10;tumor necrosis factor superfamily member 10.
• NCBI Gene Id: 8743
• Protein Name: Tumor necrosis factor ligand superfamily member 10
• Description of Target: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed:26457518, PubMed:10549288). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
• Uniprot ID: P50591
• Protein Accession #: NP_003801
• Nucleotide Accession #: NM_003810