TNFSF10 Antibody (OAEE00751)

Catalog No: OAEE00751

Size:100UG

Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5).
The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL.
A - medium
B - recombinant TRAIL
C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 ug/ml)
D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 ug/ml)
E - recombinant TRAIL + Isotype mouse IgG1 control

Product Info

• Predicted Species Reactivity: Human
• Product Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: 2E5
• Isotype: IgG1
• Host: Mouse
• Conjugation: R-PE
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Recombinant soluble fragment (aa 95-281) of human TRAIL.
• Purification: Purified by size-exclusion chromatography
• Concentration: 0.1 mg/ml
• Specificity: The antibody 2E5 reacts with TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues.
• Formulation: PE
• Storage Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
• Reference: Plasilova M, Zivny J, Jelinek J, Neuwirtova R, Cermak J, Necas E, Andera L, Stopka T: TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasiaprogenitors. Leukemia. 2002 Jan;16(1):67-73.
  Hyer ML, Croxton R, Krajewska M, Krajewski S, Kress CL, Lu M, Suh N, Sporn MB, Cryns VL, Zapata JM, Reed JC: Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res. 2005 Jun 1;65(11):4799-808.
  

Target Info

• Gene Symbol: TNFSF10
• Gene Full Name: TNF superfamily member 10
• Alias Symbols: Apo-2 ligand;Apo-2L;APO2L;CD253;chemokine tumor necrosis factor ligand superfamily member 10;TL2;TNF-related apoptosis inducing ligand TRAIL;TNF-related apoptosis-inducing ligand;TNLG6A;TRAIL;tumor necrosis factor (ligand) family, member 10;tumor necrosis factor (ligand) superfamily, member 10;tumor necrosis factor apoptosis-inducing ligand splice variant delta;tumor necrosis factor ligand 6A;tumor necrosis factor ligand superfamily member 10;tumor necrosis factor superfamily member 10.
• NCBI Gene Id: 8743
• Protein Name: Tumor necrosis factor ligand superfamily member 10
• Description of Target: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed:26457518, PubMed:10549288). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
• Uniprot ID: P50591
• Protein Accession #: NP_001177871.1; NP_003801.1
• Nucleotide Accession #: NM_001190942.1; NM_003810.3