TNFRSF18 Recombinant Protein (Human) (OPED00048)

Catalog No: OPED00048

Size:50UG

Figure: Receptor binding assay: a 96 well ELISA plate was coated O/N with 50ng GITR:Fc per well. After a blocking step, 100ul of supernatant containing transiently expressed hGITRL (diamonds) or hFasL (squares), were added to the first well. To each well were subsequently added 100ul of the previous one in a 1:1 dilution . After extensive washing, enhancer for ligands at 1ug/ml was added for 1 hour. Bound ligand was revealed by a rabbit anti-mouse IgG-HRP (1:1'000 dilution) incubated for 1 hour. OPD was used as a substrate, absorbance was measured at 490 nm in an ELISA reader.

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Lyophilized. Contains PBS.
• Host: HEK 293 cells
• Application: ELISA
• Reconstitution and Storage: Reconstitute with 50 ul sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. Store at -20C
• Purity: >90% (SDS-PAGE)
• Application Info: ELISA: binds to human GITRL.
• Reference: Identification of glucocorticoid-induced TNF receptor-related protein ligand on keratinocytes: ligation by GITR induces keratinocyte chemokine production and augments T-cell proliferation: A.M. Byrne, et al.; J. Invest. Dermatol. 129, 2784 (2009), Abstract; Full Text

Target Info

• Gene Symbol: TNFRSF18
• Gene Full Name: TNF receptor superfamily member 18
• Alias Symbols: activation-inducible TNFR family receptor;AITR;CD357;ENERGEN;GITR;GITR-D;glucocorticoid-induced TNFR-related protein;TNF receptor superfamily activation-inducible protein;tumor necrosis factor receptor superfamily member 18.
• NCBI Gene Id: 8784
• Protein Name: Tumor necrosis factor receptor superfamily member 18
• Description of Target: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
• Uniprot ID: Q9Y5U5
• Protein Accession #: NP_002417.2
• Nucleotide Accession #: NM_002426.5
• Protein Size (# AA): Recombinant
• Molecular Weight: 47 kDa