Catalog No: OKEH00366
Size:96T
Price: $525.00
SKU
OKEH00366
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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TNFRSF14 ELISA Kit (Human) : 96 Wells (OKEH00366)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TNFRSF14 ELISA Kit (Human) (OKEH00366) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human TNFRSF14 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TNFRSF14 and incubated. The TNFRSF14 found in the sample or standards competes with the biotinylated TNFRSF14 for limited binding sites on the immobilized anti-human TNFRSF14 antibody. Excess unbound biotinylated TNFRSF14 and sample or standard TNFRSF14 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TNFRSF14 captured in the well and inversely proportional to the amount of TNFRSF14 which was contained in the sample or standard.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 6.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-TNFRSF14 Microplate96 Wells (12 x 8 Well strips)
TNFRSF14 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X TNFRSF14-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity15 pg/mL
SpecificityNatural and recombinant Human Tumor necrosis factor receptor superfamily member 14
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTNFRSF14
Gene Full NameTNF receptor superfamily member 14
Alias SymbolsATAR;CD270;CD40-like protein;herpes virus entry mediator A;HVEA;HVEM;LIGHTR;TR2;tumor necrosis factor receptor superfamily member 14;tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator);Tumor necrosis factor receptor-like 2;tumor necrosis factor receptor-like gene2.
NCBI Gene Id8764
Protein NameQ92956
Description of TargetTumor necrosis factor receptor superfamily member 14
Uniprot IDhttps://www.uniprot.org/uniprot/Q92956
Protein Accession #NP_001284534.1
Nucleotide Accession #NM_001297605.1
Protein Size (# AA)283
Molecular Weight30 kDa
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