Catalog No: OKEH00366
Size:96T
Price: $525.00
SKU
OKEH00366
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology TNFRSF14 ELISA Kit (Human) (OKEH00366) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human TNFRSF14 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TNFRSF14 and incubated. The TNFRSF14 found in the sample or standards competes with the biotinylated TNFRSF14 for limited binding sites on the immobilized anti-human TNFRSF14 antibody. Excess unbound biotinylated TNFRSF14 and sample or standard TNFRSF14 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TNFRSF14 captured in the well and inversely proportional to the amount of TNFRSF14 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 31.2-2000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.1% (n = 20) Mean Inter-assay CV%: < 6.5% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 15 pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Tumor necrosis factor receptor superfamily member 14 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | TNFRSF14 |
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Gene Full Name | TNF receptor superfamily member 14 |
Alias Symbols | ATAR;CD270;CD40-like protein;herpes virus entry mediator A;HVEA;HVEM;LIGHTR;TR2;tumor necrosis factor receptor superfamily member 14;tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator);Tumor necrosis factor receptor-like 2;tumor necrosis factor receptor-like gene2. |
NCBI Gene Id | 8764 |
Protein Name | Q92956 |
Description of Target | Tumor necrosis factor receptor superfamily member 14 |
Uniprot ID | https://www.uniprot.org/uniprot/Q92956 |
Protein Accession # | NP_001284534.1 |
Nucleotide Accession # | NM_001297605.1 |
Protein Size (# AA) | 283 |
Molecular Weight | 30 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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