TNFRSF13C/BAFFR ELISA Kit (Human) (OKBB00499)

Catalog No: OKBB00499

Size:96 Wells

Human TNFRSF13C/BAFFR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human TNFRSF13C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for TNFRSF13C has been precoated onto 96-well plates. Standards(NS0, S7-A71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human TNFRSF13C amount of sample captured in plate.
• ELISA Kit Range: 78 pg/ml - 5,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	643	1604	3262	745	1862	3657
  SD	29.6	57.75	169.6	43.21	91.3	234.1
  CV%	4.6	3.6	5.2	5.8	4.9	6.4

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human TNFRSF13C Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human TNFRSF13C Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human TNFRSF13C Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human TNFRSF13C
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S7-A71
• Description: For quantitative detection of human TNFRSF13C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: TNFRSF13C
• Gene Full Name: tumor necrosis factor receptor superfamily, member 13C
• Alias Symbols: B Cell Activating Factor Receptor, B-cell-activating factor receptor, BAFF R, BAFF receptor, BAFF-R, BAFFR, BLyS receptor 3, BlySR3, BR 3, BR3, BROMIX, CD 268, CD268, CD268 antigen, CVID4, MGC138235, OTTHUMP00000028746, Prolixin, TNFRSF 13C, TNFRSF13C, TR13C_HUMAN, Tumor Necrosis Factor Receptor Subunit Member 13C, Tumor necrosis factor receptor superfamily member 13C
• NCBI Gene Id: 115650
• Protein Name: Tumor necrosis factor receptor superfamily member 13C
• Description of Target: Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
• Uniprot ID: Q96RJ3
• Protein Accession #: NP_443177.1
• Nucleotide Accession #: NM_052945.3