TNFRSF12A ELISA Kit (Human) (OKEH01840)

Catalog No: OKEH01840

Size:96T

Human TNFRSF12A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology TNFRSF12A ELISA Kit (Human) (OKEH01840) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFRSF12A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFRSF12A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFRSF12A captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.9% (n = 20)
  Mean Inter-assay CV%: < 8.4% (n = 20)
• ELISA Kit Component:
  • TNFRSF12A Microplate: 96 Wells (12 x 8 Well strips)
  • TNFRSF12A Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated TNFRSF12A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.067 ng/mL
• Specificity: Natural and recombinant Human Tumor necrosis factor receptor superfamily member 12A
• Publications:

  Biofluid quantification of TWEAK/Fn14 axis in combination with a selected biomarker panel improves assessment of prostate cancer aggressiveness. J Transl Med. 17, 307 (2019). 31500625

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for TNFRSF12A ELISA Kit (Human) (OKEH01840) on CiteAb

Target Info

• Gene Symbol: TNFRSF12A
• Gene Full Name: TNF receptor superfamily member 12A
• Alias Symbols: CD266;FGF-inducible 14;fibroblast growth factor-inducible immediate-early response protein 14;FN14;tumor necrosis factor receptor superfamily member 12A;TWEAKR;tweak-receptor;type I transmembrane protein Fn14.
• NCBI Gene Id: 51330
• Protein Name: Q9NP84
• Description of Target: Tumor necrosis factor receptor superfamily member 12A
• Uniprot ID: https://www.uniprot.org/uniprot/Q9NP84
• Protein Accession #: NP_057723.1
• Nucleotide Accession #: NM_016639.2
• Protein Size (# AA): 129
• Molecular Weight: 13 kDa