TNFRSF11B Chemi-Luminescent ELISA Kit (Mouse) (OKCD03842)

Catalog No: OKCD03842

Size:96WELLS

Mouse TNFRSF11B ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	86-101%	88-101%	88-103%	79-99%
  EDTA Plasma (n=5)	79-103%	89-98%	80-99%	78-92%
  Heparin Plasma (n=5)	90-99%	83-92%	97-104%	92-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Osteoprotegerin (OPG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Osteoprotegerin (OPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Osteoprotegerin (OPG) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-90	82
  EDTA Plasma (n=5)	83-102	93
  Heparin Plasma (n=5)	90-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteoprotegerin (OPG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteoprotegerin (OPG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Tnfrsf11b Microplate: 96 Wells (12 x 8 Well strips)
  • Tnfrsf11b Lyophilized Standard: 2
  • 100X Tnfrsf11b Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.0274 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Tnfrsf11b
• Gene Full Name: tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
• Alias Symbols: O;OC;OCIF;Opg;osteoclastogenesis inhibitory factor;osteoprotegerin;TR;TR1;tumor necrosis factor receptor superfamily member 11B.
• NCBI Gene Id: 18383
• Protein Name: Tumor necrosis factor receptor superfamily member 11B
• Description of Target: Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
• Uniprot ID: O08712
• Protein Accession #: NP_032790
• Nucleotide Accession #: NM_008764