Tnfrsf11a ELISA Kit (Mouse) (OKEH04597)

Catalog No: OKEH04597

Size:96 Wells

Mouse Tnfrsf11a ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Tnfrsf11a ELISA Kit (Mouse) (OKEH04597) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tnfrsf11a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tnfrsf11a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfrsf11a captured in well.
• ELISA Kit Range: 31.2 - 2,000 pg/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-Tnfrsf11a Microplate: 96 Wells (12 x 8 Well Strips)
  • Tnfrsf11a Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Tnfrsf11a Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: < 31.2 pg/mL
• Specificity: This assay recognizes recombinant and natural mouse TNR11. No significant cross-reactivity or interference was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TNFRSF11A
• Gene Full Name: Tumor necrosis factor receptor superfamily, member 11a, NFKB activator
• Alias Symbols: OFE, ODFR, Rank, Ly109, mRANK, TRANCE-R
• NCBI Gene Id: 21934
• Protein Name: Tumor necrosis factor receptor superfamily member 11A
• Description of Target: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.
• Uniprot ID: O35305
• Protein Accession #: NP_033425.3
• Nucleotide Accession #: NM_009399.3
• Protein Size (# AA): 625
• Molecular Weight: 67 KDa