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Catalog No: OKCA02319
Size:96 Wells
Price: $645.00
SKU
OKCA02319
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat8986-92
1:29591-99
1:49892-104
1:89282-95
ELISA Kit PrincipleAviva Systems Biology TNFRSF10D ELISA Kit (Human) (OKCA02319) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFRSF10D has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFRSF10D is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard TNFRSF10D captured in the well.
ELISA Kit Range0.312 - 20 ng/mL
ELISA Kit Recovery
Sample TypeMean Recovery (%)Range (%)
Serum (n=5)9590-101
EDTA Plasma (n=4)107102-110
ELISA Kit ReproducibilityMean Intra-Assay: CV <12% (n=20)
Mean Inter-Assay: CV <15% (n=24)
ELISA Kit Component
ComponentAmount
TNFRSF10D Microplate96 Wells (12 x 8 Well strips)
TNFRSF10D Lyophilized Standard2
100X Biotinylated TNFRSF10D Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.078 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTNFRSF10D
Alias SymbolsDCR2, CD264, TRUNDD, TRAILR4, TRAIL-R4
NCBI Gene Id8793
Protein NameTumor necrosis factor receptor superfamily member 10D
Description of TargetReceptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway.
Uniprot IDQ9UBN6
Protein Accession #NP_003831
Nucleotide Accession #NM_003840
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