TNFRSF10B ELISA Kit (Human) : 96 Wells (OKEH01884)

Catalog No: OKEH01884

Size:96T

Human TNFRSF10B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology TNFRSF10B ELISA Kit (Human) (OKEH01884) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFRSF10B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFRSF10B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFRSF10B captured in well.
• ELISA Kit Range: 0.31-20.0ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.8% (n = 20)
  Mean Inter-assay CV%: < 8.6% (n = 20)
• ELISA Kit Component:
  • TNFRSF10B Microplate: 96 Wells (12 x 8 Well strips)
  • TNFRSF10B Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated ABO Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.16 ng/mL
• Specificity: Natural and recombinant Human Tumor necrosis factor receptor superfamily member 10B
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TNFRSF10B
• Gene Full Name: TNF receptor superfamily member 10b
• Alias Symbols: apoptosis inducing protein TRICK2A/2B;apoptosis inducing receptor TRAIL-R2;CD262;cytotoxic TRAIL receptor-2;death domain containing receptor for TRAIL/Apo-2L;death receptor 5;DR5;Fas-like protein;KILLER;KILLER/DR5;p53-regulated DNA damage-inducible cell death receptor(killer);TNF-related apoptosis-inducing ligand receptor 2;TRAILR2;TRAIL-R2;TRICK2;TRICK2A;TRICK2B;TRICKB;tumor necrosis factor receptor superfamily member 10B;tumor necrosis factor receptor superfamily, member 10b;tumor necrosis factor receptor-like protein ZTNFR9;ZTNFR9.
• NCBI Gene Id: 8795
• Protein Name: O14763
• Description of Target: Tumor necrosis factor receptor superfamily member 10B
• Uniprot ID: https://www.uniprot.org/uniprot/O14763
• Protein Accession #: NP_003833.4
• Nucleotide Accession #: NM_003842.4
• Protein Size (# AA): 440
• Molecular Weight: 48 kDa