TNFAIP1 Antibody (OABB00264)

Catalog No: OABB00264 (Formerly GWB-BBP185)

Size:100UG

Anti-TNFAIP1 antibody, OABB00264, Western blotting
All lanes: Anti TNFAIP1(OABB00264) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD

IHC(P): Human Rectal Cancer Tissue

ICC: Hela Cell

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunocytochemistry|Immunohistochemistry|Immunohistochemistry-Frozen|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Tumor necrosis factor, alpha-induced protein 1(endothelial), also known as TNFAIP1, is a human gene. The gene, present in single copy, was located in the 17q22-q23 region. This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha(TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. The protein is involved in the primary response of the endothelium to TNF.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human, Rat: Mouse
  Immunocytochemistry : 0.5-1 ug/ml: Human, Rat: Mouse
• Reference: 1. Wolf, F. W.; Marks, R. M.; Sarma, V.; Byers, M. G.; Katz, R. W.; Shows, T. B.; Dixit, V. M. : Characterization of a novel tumor necrosis factor-alpha-induced endothelial primary response gene. J. Biol. Chem. 267: 1317-1326, 1992.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2(TNFAIP1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

Target Info

• Gene Symbol: TNFAIP1
• Gene Full Name: TNF alpha induced protein 1
• Alias Symbols: B12;B61;BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2;BTB/POZ domain-containing protein TNFAIP1;BTBD34;EDP1;hBACURD2;Protein B12;tumor necrosis factor, alpha induced protein 1;tumor necrosis factor, alpha-induced protein 1 (endothelial);Tumor necrosis factor, alpha-induced protein 1, endothelial.
• NCBI Gene Id: 7126
• Protein Name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• Description of Target: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.
• Uniprot ID: Q13829
• Protein Accession #: NP_066960.1
• Molecular Weight: 36204 MW