Tnf Recombinant Protein (Mouse) (OPED00139)

Catalog No: OPED00139

Size:50UG

SDS-PAGE Analysis of TNF-α (soluble) (mouse), (recombinant). Lane 1: MW Marker, Lane 2: 1 ug TNF-α

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Product Format: Lyophilized. Contains PBS.
• Host: E. Coli
• Application: ELISA
• Additional Information: Historical lots have shown that it exerts its biological activity in a concentration range of 0.5-1ng/ml (WEHI 164 cells).
• Reconstitution and Storage: Reconstitute with 50 ul sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. Store at -20C
• Purity: >90% (SDS-PAGE)
• Application Info: ELISA: binds to human TNF-R1 receptor.

Target Info

• Reference: A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood 117, 5953 (2011), Application(s): Death assay on mouse neutrophils, Abstract; Full Text
  Inhibition of interleukin 1 receptor/Toll-like receptor signaling through the alternatively spliced, short form of MyD88 is due to its failure to recruit IRAK-4: K. Burns, et al.; J. Exp. Med. 197, 263 (2003), Abstract; Full Text
  Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998), Abstract; Full Text
  General Literature References
  The transmembrane form of tumor necrosis factor is the prime activating ligand of the 80 kDa tumor necrosis factor receptor: M. Grell, et al.; Cell 83, 793 (1995), Abstract;
  The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death: C.A. Smith, et al.; Cell 76, 959 (1994), Abstract;
  Tumor necrosis factor: a pleiotropic cytokine and therapeutic target: K.J. Tracey & A. Cerami; Annu. Rev. Med. 45, 491 (1994), Abstract;
• Gene Symbol: Tnf
• Gene Full Name: tumor necrosis factor
• Alias Symbols: cachectin;DI;DIF;Tn;TNF-;Tnfa;TNF-a;TNFalpha;TNF-alpha;Tnfs;Tnfsf1a;TNFSF2;Tnlg1f;tumor necrosis factor;tumor necrosis factor ligand 1f;tumor necrosis factor ligand superfamily member 2;tumor necrosis factor-alpha.
• NCBI Gene Id: 21926
• Protein Name: Tumor necrosis factor
• Description of Target: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
• Uniprot ID: P06804
• Protein Accession #: NP_854111.1
• Nucleotide Accession #: NC_002945.3
• Protein Size (# AA): Recombinant
• Molecular Weight: 20 kDa