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TNF ELISA Kit (Rat) : 96 Wells (OKEH00223)

96 Wells
$339.00
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Predicted Species Reactivity:
Rat
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology TNF ELISA Kit (Rat) (OKEH00223) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for TNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNF captured in well.
Kit Range:
15.6 - 1000pg/mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Kit Reproducibility:
Mean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 9.5% (n = 20)
Kit Component:
ComponentAmount
TNF Microplate96 Wells (12 x 8 Well strips)
TNF Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated TNF Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
TNF
Official Gene Full Name:
tumor necrosis factor
Alias Symbols:
Cachectin, MGC124630, RATTNF, Tnfa, TNF-a, TNF-alpha, Tnfsf2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2
NCBI Gene Id:
24835
Protein Name:
Tumor necrosis factor
Description of Target:
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Swissprot Id:
P16599
Protein Accession #:
NP_036807.1
Nucleotide Accession #:
NM_012675.3
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
7.84 pg/mL
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TNF.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TNF.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Rat Tumor necrosis factor
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
26 kDa
Protein Size (# AA):
235

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