Size:96 Wells
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TNF ELISA Kit (Human) : 96 Wells (OKAA00027-96W)

Catalog#: OKAA00027-96W
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Reproducibility The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component
2 vials human TNF-alpha Standard lyophilized, 4000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human TNF-alpha antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human TSLP monoclonal antibody
Additional Information Sample Type: cell culture supernates, serum, and plasma
:: Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human TNF-?(8x12)
2. 2 vials human TNF-? Standard lyophilized, 4000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human TNF-? polyclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Sample Type cell culture, supernates, serum, and plasma
Sensitivity 7 pg/ml
Predicted Homology Based on Immunogen Sequence Human
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay recognizes both natural and recombinant human TNF-a. The factors listed below were prepared at 70ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: TNF-b, GM-CSF, s TNF RI, s TNF RII, TNF-b,
Recombinant mouse: TNF-a, TNF-a(truncated), s TNF RI,
Recombinant porcine: TNF-a,
Assay Info Assay Method: Quantitative Sandwich ELISA
Gene Symbol TNF
Official Gene Full Name tumor necrosis factor
Alias Symbols Cachectin, DIF, TNFA, TNF-a, TNF-alpha, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2
NCBI Gene Id 7124
Protein Name Tumor necrosis factor
Description of Target This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Swissprot Id P01375
Protein Accession # NP_000585.2
Nucleotide Accession # NM_000594.3
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express TNF.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express TNF.
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