TNF ELISA Kit (Human) : 96 Wells (OKAA00027-96W)

Catalog No: OKAA00027-96W

Size:96 Wells

Standard curve of TNF protein using OKAA00027_96W

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Reproducibility: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
• ELISA Kit Component:

  Components
  2 vials human TNF-alpha Standard lyophilized, 4000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human TNF-alpha antibody
  2 vials Streptavidin-HRP solution,
  1 bottle Standard /sample Diluent
  1 bottle Biotin-Conjugate antibody Diluent
  1 bottle Streptavidin-HRP Diluent
  1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  1 vial Substrate Solution
  1 vial Stop Solution
  2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human TSLP monoclonal antibody

• Additional Information: Sample Type: cell culture supernates, serum, and plasma
  Reagents: REAGENTS
  1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human TNF-?(8x12)
  2. 2 vials human TNF-? Standard lyophilized, 4000 pg/ml upon reconstitution
  3. 2 vials concentrated Biotin-Conjugate anti-human TNF-? polyclonal antibody
  4. 2 vials Streptavidin-HRP solution,
  5. 1 bottle Standard /sample Diluent
  6. 1 bottle Biotin-Conjugate antibody Diluent
  7. 1 bottle Streptavidin-HRP Diluent
  8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  9. 1 vial Substrate Solution
  10. 1 vial Stop Solution
  11. 3 pieces Adhesive Films
  12. package insert
• Sample Type: cell culture, supernates, serum, and plasma
• Sensitivity: 7 pg/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: This assay recognizes both natural and recombinant human TNF-a. The factors listed below were prepared at 70ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
  Recombinant human: TNF-b, GM-CSF, s TNF RI, s TNF RII, TNF-b,
  Recombinant mouse: TNF-a, TNF-a（truncated）, s TNF RI,
  Recombinant porcine: TNF-a,
• Assay Info: Assay Method: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TNF
• Gene Full Name: tumor necrosis factor
• Alias Symbols: DIF, TNFA, TNFSF2, TNLG1F, TNF-alpha
• NCBI Gene Id: 7124
• Protein Name: Tumor necrosis factor
• Description of Target: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
• Uniprot ID: P01375
• Protein Accession #: NP_000585.2
• Nucleotide Accession #: NM_000594.3