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Catalog No: OKCD03984
Size:96WELLS
Price: $837.00
SKU
OKCD03984
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TNF Chemi-Luminescent ELISA Kit (Rat) (OKCD03984)

Datasheets/ManualsPrintable datasheet for TNF Chemi-Luminescent ELISA Kit (Rat) (OKCD03984)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)86-103%91-101%94-101%97-105%
EDTA Plasma (n=5)93-105%90-98%87-94%93-101%
Heparin Plasma (n=5)87-104%95-105%80-95%86-94%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha (TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tumor Necrosis Factor Alpha (TNFa) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)88-9991
EDTA Plasma (n=5)88-10399
Heparin Plasma (n=5)94-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha (TNFa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha (TNFa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Tnf Microplate96 Wells (12 x 8 Well strips)
Tnf Lyophilized Standard2
100X Tnf Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity1.37 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLOC103694380|Tnf
Gene Full Nametumor necrosis factor|tumor necrosis factor-like
Alias Symbolscachectin;RATTNF;Tnfa;TNF-alpha;tumor necrosis factor;tumor necrosis factor (TNF superfamily, member 2);tumor necrosis factor ligand superfamily member 2.
NCBI Gene Id103694380|24835
Protein NameTumor necrosis factor
Description of TargetCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Uniprot IDP16599
Protein Accession #NP_036807
Nucleotide Accession #NM_012675
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