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Tnf Antibody (OASA08717)

0.1 mg
$609.00
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Gene Symbol:
Tnf
NCBI Gene Id:
24835
Protein Name:
Tumor necrosis factor
Swissprot Id:
P16599
Protein Accession #:
NP_036807.1
Alias Symbols:
Tnfa, RATTNF, MGC124630, TNF-alpha, Tnf
Replacement Item:
This antibody may replace item sc-101425 from Santa Cruz Biotechnology.
Description of Target:
RABBIT ANTI RAT TNF ALPHA
Protein Size (# AA):
235
Host:
Rabbit
Clonality:
Polyclonal
Application:
ELISA, IHC-FFPE, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Tnf.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Tnf.
Immunogen:
The immunogen for anti-Tnf antibody: recombinant rat TNF alpha
Predicted Species Reactivity:
Rat
Predicted Homology Based on Immunogen Sequence:
Rat
Product Format:
Purified IgG - lyophilised
Reconstitution and Storage:
Reconstitution: Reconstitute with 0.1 ml distilled water
Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-Tnf antibody - OASA08717
Specificity:
TNF ALPHA
Isotype:
Polyclonal IgG
Application Info:
ELISA:    0.5ug/ml
Western Blotting:    0.1 - 0.2ug/ml
Functional Assays:    0.145 - 0.165ug/ml
Additional Information:
Note: The addition of 0.09% sodium azide is recommended for long term storage.
N.B. For functional studies do not add sodium azide.
:::
Preservative Stabilisers: None present
Antiserum Preparation: Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Halin, S. et al. (2010) Pigment epithelium-derived factor stimulates tumor macrophage recruitment and is
downregulated by the prostate tumor microenvironment. Neoplasia. 12: 336-45.

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