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Tnf Antibody (OASA05777)

  • Catalog#: OASA05777
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Gene Symbol:
Tnf
NCBI Gene Id:
21926
Protein Name:
Tumor necrosis factor
Swissprot Id:
P06804
Protein Accession #:
NP_038721.1
Alias Symbols:
DIF, Tnfa, TNFSF2, Tnfsf1a, TNFalpha, MGC151434, TNF-alpha, Tnf
Description of Target:
RAT ANTI MOUSE TNF ALPHA:Biotin
Protein Size (# AA):
235
Host:
Rat
Clonality:
Monoclonal
Application:
ELISA, FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Tnf.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Tnf.
Immunogen:
The immunogen for anti-Tnf antibody: recombinant murine TNF alpha expressed in E. coli
Predicted Species Reactivity:
Mouse
Predicted Homology Based on Immunogen Sequence:
Mouse
Product Format:
Purified IgG conjugated to Biotin - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-Tnf antibody - OASA05777
Specificity:
TNF ALPHA
Clone:
MP6-XT22
Conjugation:
Biotin
Isotype:
IgG1
Application Info:
ELISA : This antibody may be used as a detection reagent in a sandwich ELISA assay for murine TNF using clone MP6-XT3 (OASA05774) as capture reagent.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106
Application Data:
Application #1: Recombinant mouse TNF alpha detected with Rat anti Mouse TNF alpha
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
1% - Bovine Serum Albumin
:::
Approx Protein Conc: IgG concentration 0.5 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Abrams, J. S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples Immunol. Rev. 127: 5-24.
2. Hostager, B. et al. (2002) Role of TNF receptor-associated factor 2 in the activation of IgM secretion by CD40 and CD120b. J. Immunol. 168:3318-3322.
3. Ross, S. et al. (1997) Suppression of TNF-alpha expression, inhibition of Th1 activity, and amelioration of collagen-induced arthritis by rolipram. J. Immunol. 159:6253-6259.
4. Scanga, C. et al. (1999) Reactivation of latent tuberculosis : variations on the Cornell murine model. Infect. Immun. 67:4531-4538.
5. Yang, J. et al. (1995) Involvement of natural killer cells in nitric oxide production by spleen cells after stimulation with Mycobacterium bovis BCG. Study of the mechanism of the different abilities of viable and killed BCG. J. Immunol. 155:5728-5735.

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