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TNF Antibody - Biotin Conjugated (OASA00368)

0.25 mg
$585.00
In Stock
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Gene Symbol:
TNF
NCBI Gene Id:
280943
Protein Name:
Tumor necrosis factor
Swissprot Id:
Q06599
Protein Accession #:
NP_776391.2
Alias Symbols:
TNFa
Replacement Item:
This antibody may replace item sc-101425 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI BOVINE TNF ALPHA:Biotin
Protein Size (# AA):
234
Host:
Mouse
Clonality:
Monoclonal
Application:
ELISA, FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TNF.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TNF.
Immunogen:
The immunogen for anti-TNF antibody: recombinant bovine TNF alpha
Predicted Species Reactivity:
Cow
Predicted Homology Based on Immunogen Sequence:
Cow
Product Format:
Purified IgG conjugated to Biotin - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-TNF antibody - OASA00368
Specificity:
TNF ALPHA
Clone:
CC328
Conjugation:
Biotin
Isotype:
IgG2a
Application Info:
ELISA : This biotin conjugate may be used as a detection antibody in sandwich ELISA assays for bovine TNF alpha in combination with OASA00367 as capture reagent.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Hope, J.C. et al. (2003) Maturation of bovine dendritic cells by lipopeptides. Vet. Immunol. Immunopathol. 95: 21-31.
2. Whelan, A.O. et al. (2003) Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide. Infect. Immun. 71(11): 6420-6425.
3. Guergnon, J. et al. (2003) A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaB activation of Theileria parva-transformed B cells. Cell. Microbiol. 5(10): 709-716.
4. Kwong, L.S. et al. (2010) Production and characterization of two monoclonal antibodies to bovine tumour

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