- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for TNF Antibody (OAAF02103) Please click here to view the MSDS. |
---|
Predicted Species Reactivity | Human|Mouse|Rat |
---|---|
Product Format | Liquid PBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human TNFA. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen. |
Concentration | 1 mg/ml |
Specificity | TNFA antibody detects endogenous levels of total TNFA protein. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~100 IF: 1:100~500 ELISA: 1:10000 |
Reference | Neville M.J., J. Immunol. 162:4745-4754(1999). Xie T., Genome Res. 13:2621-2636(2003). Kim Y.J., Hum. Mol. Genet. 12:2541-2546(2003). |
Gene Symbol | TNF |
---|---|
Gene Full Name | tumor necrosis factor |
Alias Symbols | APC1 protein;Cachectin;DIF;TNF, macrophage-derived;TNF, monocyte-derived;TNF-a;TNFA;TNF-alpha;TNFSF2;TNLG1F;tumor necrosis factor;tumor necrosis factor ligand 1F;tumor necrosis factor ligand superfamily member 2;tumor necrosis factor-alpha. |
NCBI Gene Id | 7124 |
Protein Name | Tumor necrosis factor |
Description of Target | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918). |
Uniprot ID | P01375 |
Protein Accession # | NP_000585.2 |
Molecular Weight | 25 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "TNF Antibody (OAAF02103)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
-
How long will it take to receive "TNF Antibody (OAAF02103)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
-
What buffer format is "TNF Antibody (OAAF02103)" provided in?
This item is provided in "Liquid PBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol ".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "TNF Antibody (OAAF02103)"?
This target may also be called "APC1 protein;Cachectin;DIF;TNF, macrophage-derived;TNF, monocyte-derived;TNF-a;TNFA;TNF-alpha;TNFSF2;TNLG1F;tumor necrosis factor;tumor necrosis factor ligand 1F;tumor necrosis factor ligand superfamily member 2;tumor necrosis factor-alpha." in publications.
-
What is the shipping cost for "TNF Antibody (OAAF02103)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "TNF Antibody (OAAF02103)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "TNF Antibody (OAAF02103)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "25 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "TNF Antibody (OAAF02103)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "TNF"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "TNF"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "TNF"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "TNF"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "TNF"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "TNF"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.