Size:10 ug
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TNF ALPHA Recombinant Protein (OPSA10958)

Catalog#: OPSA10958
This product is Genway GWB-18E6EF
More Information
Predicted Species Reactivity Human
Product Format Purified recombinant cytokine - lyophilised
Additional Information Approx Protein Conc: Total protein concentration 0.1 mg/ml after reconstitution
Buffer solution: TRIS buffered saline
:: Endotoxin Level: < 0.1ng/ug
:: Preservative: None present
Reconstitution and Storage Reconstitution: Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva Systems Biology recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide.
Storage: Prior to reconstitution store at +4C. Following reconstitution store at -20C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Predicted Homology Based on Immunogen Sequence Human
Purity >98% by SDS PAGE and HPLC analysis
Biological Activity This product has a specific activity of >2 x 107 units/mg as determined by cytolysis of L929 cells in the presence of Actinomycin D.
Application Info Suggested Dilutions:
ELISA: 0.2-0.4 ng/well
Western Blot: 1.5-3.0 ng/lane
Functional Assays: 0.05 ng/ml -20 ng/ml
This product may be used as a standard in ELISA with either OASA06477 or OASA06478.
This product may be used as the positive control in Western Blotting applications with either OASA06477 or OASA06478.
Target Reference 1. Cernuda-Morollon, E. et al. (2001) 15-Deoxy-Delta 12,14-prostaglandin J2 inhibition of NF-kappaB-DNA binding through covalent modification of the p50 subunit. J. Biol.Chem. 276 (38): 35530 - 35536
2. Taubert, A. & Hermosilla, C. (2008) Bovine recombinant IFNgamma induces endothelial cell gene transcription of immunoregulatory molecules and upregulates PMN and PBMC adhesion on bovine endothelial cells. Vet Res Commun. 32: 35-47.
Datasheets/Manuals Printable datasheet for OPSA10958
Gene Symbol TNF
Alias Symbols DIF, TNFA, TNFSF2, TNF-alpha, TNF
NCBI Gene Id 7124
Protein Name Tumor necrosis factor
Swissprot Id P01375
Protein Accession # NP_000585.2
Protein Size (# AA) 233
Molecular Weight 17.4 kD based on 157 amino acid sequence
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express TNF.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express TNF.
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