TNF ALPHA Recombinant Protein (OPSA10958)
This product is Genway GWB-18E6EF
|Predicted Species Reactivity||Human|
|Product Format||Purified recombinant cytokine - lyophilised|
|Additional Information||Approx Protein Conc: Total protein concentration 0.1 mg/ml after reconstitution
Buffer solution: TRIS buffered saline
|::||Endotoxin Level: < 0.1ng/ug|
|::||Preservative: None present|
|Reconstitution and Storage||Reconstitution: Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva Systems Biology recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide.
Storage: Prior to reconstitution store at +4C. Following reconstitution store at -20C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
|Predicted Homology Based on Immunogen Sequence||Human|
|Purity||>98% by SDS PAGE and HPLC analysis|
|Biological Activity||This product has a specific activity of >2 x 107 units/mg as determined by cytolysis of L929 cells in the presence of Actinomycin D.|
|Application Info||Suggested Dilutions:
ELISA: 0.2-0.4 ng/well
Western Blot: 1.5-3.0 ng/lane
Functional Assays: 0.05 ng/ml -20 ng/ml
This product may be used as a standard in ELISA with either OASA06477 or OASA06478.
This product may be used as the positive control in Western Blotting applications with either OASA06477 or OASA06478.
|Target Reference||1. Cernuda-Morollon, E. et al. (2001) 15-Deoxy-Delta 12,14-prostaglandin J2 inhibition of NF-kappaB-DNA binding through covalent modification of the p50 subunit. J. Biol.Chem. 276 (38): 35530 - 35536
2. Taubert, A. & Hermosilla, C. (2008) Bovine recombinant IFNgamma induces endothelial cell gene transcription of immunoregulatory molecules and upregulates PMN and PBMC adhesion on bovine endothelial cells. Vet Res Commun. 32: 35-47.
|Datasheets/Manuals||Printable datasheet for OPSA10958|
|Alias Symbols||DIF, TNFA, TNFSF2, TNF-alpha, TNF|
|NCBI Gene Id||7124|
|Protein Name||Tumor necrosis factor|
|Description of Target||RECOMBINANT HUMAN TNF ALPHA
|Protein Accession #||NP_000585.2|
|Protein Size (# AA)||233|
|Molecular Weight||17.4 kD based on 157 amino acid sequence|
|Tissue Tool||Find tissues and cell lines supported by DNA array analysis to express TNF.|
|RNA Seq||Find tissues and cell lines supported by RNA-seq analysis to express TNF.|
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
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