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Catalog No: OKCA01940
Size:96 Wells
Price: $754.00
SKU
OKCA01940
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
ELISA Kit PrincipleAviva Systems Biology Tmsb4x ELISA Kit (Rat) (OKCA01940) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tmsb4x has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tmsb4x is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Tmsb4x captured in the well.
ELISA Kit Range62.5 - 4,000 ng/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)8984-93
EDTA Plasma (n=4)9590-100
ELISA Kit Component
ComponentAmount
Tmsb4x Microplate96 Wells (12 x 8 Well strips)
Tmsb4x Lyophilized Standard2 x 4 ug
100X Biotinylated Tmsb4x Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity15.6 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolTMSB4X
Gene Full Namethymosin beta 4, X-linked
Alias SymbolsFX, PTMB4, TB4X, TMSB4, OTTHUMP00000022925|OTTHUMP00000022927|OTTHUMP00000022928|prothymosin beta-4|thymosin beta-4|thymosin, beta 4|thymosin, beta 4, X chromosome
NCBI Gene Id81814
Protein NameThymosin beta-4
Description of TargetPlays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.Seraspenide inhibits the entry of hematopoietic pluripotent stem cells into the S-phase.
Uniprot IDP62329
Protein Accession #NP_112398.1
Nucleotide Accession #NM_031136.1
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