Catalog No: OKCA01940
Size:96 Wells
Price: $754.00
SKU
OKCA01940
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Rat | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology Tmsb4x ELISA Kit (Rat) (OKCA01940) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tmsb4x has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tmsb4x is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Tmsb4x captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 62.5 - 4,000 ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
Sensitivity | 15.6 ng/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sanadwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | TMSB4X |
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Gene Full Name | thymosin beta 4, X-linked |
Alias Symbols | FX, PTMB4, TB4X, TMSB4, OTTHUMP00000022925|OTTHUMP00000022927|OTTHUMP00000022928|prothymosin beta-4|thymosin beta-4|thymosin, beta 4|thymosin, beta 4, X chromosome |
NCBI Gene Id | 81814 |
Protein Name | Thymosin beta-4 |
Description of Target | Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.Seraspenide inhibits the entry of hematopoietic pluripotent stem cells into the S-phase. |
Uniprot ID | P62329 |
Protein Accession # | NP_112398.1 |
Nucleotide Accession # | NM_031136.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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