TMSB4X ELISA Kit (Mouse) (OKEH01277)

Catalog No: OKEH01277

Size:96T

Mouse TMSB4X ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology TMSB4X ELISA Kit (Mouse) (OKEH01277) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse TMSB4X antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TMSB4X and incubated. The TMSB4X found in the sample or standards competes with the biotinylated TMSB4X for limited binding sites on the immobilized anti-mouse TMSB4X antibody. Excess unbound biotinylated TMSB4X and sample or standard TMSB4X is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TMSB4X captured in the well and inversely proportional to the amount of TMSB4X which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.9% (n = 20)
  Mean Inter-assay CV%: < 8.3% (n = 20)
• ELISA Kit Component:
  • Anti-TMSB4X Microplate: 96 Wells (12 x 8 Well strips)
  • TMSB4X Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X TMSB4X-Biotin Complex: 1 x 60 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.168 ng/mL
• Specificity: Natural and recombinant Mouse Thymosin beta-4
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Tmsb4x
• Gene Full Name: thymosin, beta 4, X chromosome
• Alias Symbols: prothymosin beta 4;Ptmb;Ptmb4;t beta 4;Tb4;Tbeta;Tbeta4;thymosin beta-4.
• NCBI Gene Id: 19241
• Protein Name: P20065
• Description of Target: Thymosin beta-4
• Uniprot ID: https://www.uniprot.org/uniprot/P20065
• Protein Accession #: NP_067253.1
• Nucleotide Accession #: NM_021278.2
• Protein Size (# AA): 50
• Molecular Weight: 6 kDa