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Catalog No: OKCD00599 (Formerly GWB-KBBPZ3)
Size:96WELLS
Price: $675.00
SKU
OKCD00599
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Datasheets/ManualsPrintable datasheet for TMEM27 ELISA Kit (Human) (OKCD00599)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protein 27 (TMEM27). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protein 27 (TMEM27). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protein 27 (TMEM27), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transmembrane Protein 27 (TMEM27) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transmembrane Protein 27 (TMEM27) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transmembrane Protein 27 (TMEM27) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TMEM27 Microplate96 Wells (12 x 8 Well Strips)
TMEM27 Lyophilized Standard2 x 10 ng
100X Biotinylated TMEM27 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.056 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Transmembrane Protein 27 (TMEM27). No significant cross-reactivity or interference between Transmembrane Protein 27 (TMEM27) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCLTRN
Gene Full Namecollectrin, amino acid transport regulator
Alias Symbolscollectrin;kidney-specific membrane protein;NX17;NX-17;TMEM27;transmembrane protein 27.
NCBI Gene Id57393
Protein NameCollectrin
Description of TargetRegulator of SNARE complex function. Stimulator of beta cell replication.
Uniprot IDQ9HBJ8
Protein Accession #NP_065716
Nucleotide Accession #NM_020665.5
Protein Size (# AA)222
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