TMB ELISA PEROXIDASE SUBSTRATE (OORA01684)

Catalog No: OORA01684

Size:1L

Product Info

• Product Format: Liquid Proprietary buffer with enhancer
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay
• Additional Information: TMB ELISA Peroxidase Substrate specifications:
  pH: 4.0 +/- 0.2
  Stability @ 18 to 26°C: PASS
  Stability @ 4°C: PASS
  QC Raw Material: PASS
  Absorbance check of final product: PASS
  Performance Data per ELISA: PASS
• Reconstitution and Storage: Store container at 4°C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required. Expiration date is one (1) year from date of receipt.
• Concentration: 1X
• Application Info: TMB ELISA Peroxidase Substrate comes ready to use. No dilutions are required. TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions.
• Publications:

  Differential internalin A levels in biofilms of Listeria monocytogenes grown on different surfaces and nutrient conditions. Int. J. Food Microbiol. 219, 50-5 (2016). 26724402

• Description: TMB ELISA Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction can be halted with acid to change the TMB to yellow.

Target Info

• Alias Symbols: 3,3',5,5'-Tetramethylbenzidine, TMB ELISA substrate, TMBE Substrate, chromogenic ELISA substrate