TLR9 Chemi-Luminescent ELISA Kit (Human) (OKCD05552)

Catalog No: OKCD05552

Size:96WELLS

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 9 (TLR9). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Toll Like Receptor 9 (TLR9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Toll Like Receptor 9 (TLR9) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 9 (TLR9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 9 (TLR9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • TLR9 Microplate: 96 Wells (12 x 8 Well strips)
  • TLR9 Lyophilized Standard: 2
  • 100X TLR9 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 4.8pg/mL

Target Info

• Gene Symbol: TLR9
• Gene Full Name: toll like receptor 9
• Alias Symbols: CD289;toll-like receptor 9.
• NCBI Gene Id: 54106
• Protein Name: Toll-like receptor 9
• Description of Target: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:11564765, PubMed:17932028). Controls lymphocyte response to Helicobacter infection (By similarity). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (PubMed:23857366).
• Uniprot ID: Q9NR96
• Protein Accession #: NP_059138.1
• Nucleotide Accession #: NM_017442.3