TLR4 ELISA Kit (Rat) : 96 Wells (OKEH00569)

Catalog No: OKEH00569

Size:96T

Rat TLR4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology TLR4 ELISA Kit (Rat) (OKEH00569) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TLR4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TLR4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TLR4 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.8% (n = 20)
  Mean Inter-assay CV%: < 8.1% (n = 20)
• ELISA Kit Component:
  • Anti-TLR4 Microplate: 96 Wells (12 x 8 Well strips)
  • TLR4 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated TLR4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.159 ng/mL
• Specificity: Natural and recombinant Rat Toll-like receptor 4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Tlr4
• Gene Full Name: toll-like receptor 4
• Alias Symbols: toll4;toll-like receptor 4.
• NCBI Gene Id: 29260
• Protein Name: Q9QX05
• Description of Target: Toll-like receptor 4
• Uniprot ID: https://www.uniprot.org/uniprot/Q9QX05
• Protein Accession #: NP_003257.1
• Nucleotide Accession #: NM_003266.3
• Protein Size (# AA): 839
• Molecular Weight: 95 kDa