TLR2-2 ELISA Kit (Chicken) (OKWB00130)

Catalog No: OKWB00130

Size:96 Wells

Chicken TLR2-2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Chicken
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	91-101%	87-104%	92-105%	85-105%
  EDTA Plasma (n=5)	85-98%	91-101%	84-100%	86-101%
  Heparin Plasma (n=5)	87-98%	80-98%	84-98%	81-97%

• ELISA Kit Principle: Aviva Systems Biology Tlr2-2 ELISA Kit (Chicken) (OKWB00130) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tlr2-2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tlr2-2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tlr2-2 captured in the well.
• ELISA Kit Range: 0.313 - 20 ng/mL
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	89-103	97
  EDTA Plasma (n=5)	87-102	97
  Heparin Plasma (n=5)	88-104	99

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Tlr2-2 Microplate: 96 Wells (12 x 8 Well strips)
  • Tlr2-2 Lyophilized Standard: 2 x 20 ng
  • 100X Tlr2-2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.188 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TLR2-2
• Alias Symbols: chTLR2, TLR2, TLR2-2, TLR2A, Toll-like receptor 2 type-1, Toll-like receptor 2 type-2
• Protein Name: Toll-like receptor 2 type-2
• Description of Target: Participates in the innate immune response to microbial agents. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mediates the response to mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2).
• Uniprot ID: Q9DGB6