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Catalog No: OKCD02072 (Formerly GWB-KBBMX6)
Size:96WELLS
Price: $750.00
SKU
OKCD02072
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Datasheets/ManualsPrintable datasheet for TLL1 ELISA Kit (Human) (OKCD02072)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)83-97%94-104%79-90%91-101%
EDTA Plasma (n=5)82-96%78-90%82-94%83-102%
Heparin Plasma (n=5)95-103%80-102%89-97%89-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tolloid Like Protein 1 (TLL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tolloid Like Protein 1 (TLL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tolloid Like Protein 1 (TLL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tolloid Like Protein 1 (TLL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)79-9890
EDTA Plasma (n=5)85-10195
Heparin Plasma (n=5)89-9793
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tolloid Like Protein 1 (TLL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tolloid Like Protein 1 (TLL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TLL1 Microplate96 Wells (12 x 8 Well Strips)
TLL1 Lyophilized Standard2 x 40 ng
100X Biotinylated TLL1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.216 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Tolloid Like Protein 1 (TLL1). No significant cross-reactivity or interference between Tolloid Like Protein 1 (TLL1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTLL1
Gene Full Nametolloid like 1
Alias SymbolsASD6;TLL;tolloid-like protein 1.
NCBI Gene Id7092
Protein NameTolloid-like protein 1
Description of TargetProtease which processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Required for the embryonic development. Predominant protease, which in the development, influences dorsal-ventral patterning and skeletogenesis.
Uniprot IDO43897
Protein Accession #NP_001191689.1
Nucleotide Accession #NM_001204760.1
Protein Size (# AA)1013
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