TLK1 Antibody - C-terminal region (OAAB17466)

Catalog No: OAAB17466

Size:400ul

Western blot analysis of hTLK1-L687 (Cat. # OAAB17466) in K562 cell line lysates (35ug/lane). TLK1 (arrow) was detected using the purified Pab.

TLK1 is strongly supported by BioGPS gene expression data to be expressed in K562

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: TLK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S- phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'
  Cellular Location: Nucleus.
  Tissue Location: Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This TLK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 720-750 amino acids from the C-terminal region of human TLK1.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  TLK1 is strongly supported by BioGPS gene expression data to be expressed in K562

• Application Info: WB: 1:8000
  WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: TLK1
• Alias Symbols: PKU-beta
• NCBI Gene Id: 9874
• Protein Name: Serine/threonine-protein kinase tousled-like 1
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9UKI8
• Protein Accession #: NP_001130026.1
• Molecular Weight: 86700 Da