TJP2 Antibody - C-terminal region (OABB01122)

Catalog No: OABB01122

Size:100UG

Anti-TJP2 antibody, OABB01122, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: PC-12 Cell Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: HEPA Cell Lysate

Anti-TJP2 antibody, OABB01122, IHC(P)
IHC(P): Mouse Liver Tissue

Product Info

• Tested Species Reactivity: Mouse, Rat
• Predicted Species Reactivity: Hamster, Mouse, Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry, Western blot
• Additional Information: Notes: WB: The detection limit for TJP2 is approximately 2.5ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: TJP2(Tight Junction Protein 2), also known as Zona Occludens 2 or ZO2, is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins(TJPs) belong to a family of membrane-associated guanylate kinase(MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. Duclos et al.(1994) mapped the TJP2 gene telomeric to the Friedreich ataxia critical region on chromosome 9q13-q21. TJP2 lies about 70 kb centromeric to the X123 gene and is transcribed in the centromere-to-telomere direction. Using in vitro assays and immunoprecipitation studies, Itoh et al.(1999) showed that the mouse Tjp1, Tjp2, and Tjp3 PDZ1 domains interacted with the C-terminal cytoplasmic domains of Cldn1 through Cldn8. In the mouse inner ear, Walsh et al.(2010) found that Tjp2 expression decreased rapidly between E16.5 and age 1 week to a level in adult mice that was approximately 50% of the level at birth(P0).
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse TJP2 (1149-1167aa AHSKRGYYSQPSRYRDTEL), identical to the related rat sequence.
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Mouse, Rat: By Heat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Tight junction protein ZO-2(TJP2) detection. Tested with WB, IHC-P in Mouse;Rat.

Target Info

• Gene Symbol: Tjp2
• Gene Full Name: tight junction protein 2
• Alias Symbols: Tight junction protein 2;tight junction protein ZO-2;Tight junction protein ZO-2 (Zonula occludens 2 protein) (Zona occludens 2 protein) (Tight junction protein 2);ZO-;ZO-2;zona occludens protein 2;zonula occludens protein 2.
• NCBI Gene Id: 21873
• Protein Name: Q9Z0U1
• Description of Target: Tight junction protein ZO-2
• Uniprot ID: https://www.uniprot.org/uniprot/Q9Z0U1
• Molecular Weight: 131280 MW