Size:96 Wells
$600.00
In Stock
SKU
OKCA01006
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TJP1 ELISA Kit (Rat) (OKCA01006)

Catalog#: OKCA01006
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species ReactivityRat
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3.5 Hours
Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9186-95
1:210297-107
1:49185-97
1:89791-103
Kit PrincipleAviva Systems Biology TJP1 ELISA Kit (Rat) (OKCA01006) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for TJP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TJP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TJP1 captured in well.
Kit Range31.2 pg/mL-2,000 pg/mL
Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9589-98
EDTA Plasma (n=4)9790-100
Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level TJP1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level TJP1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Component
ComponentAmount
TJP1 Microplate96 Wells (12 x 8 Well strips)
TJP1 Lyophilized Standard2
100X Biotinylated TJP1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity7.8 pg/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTJP1
Official Gene Full NameTight junction protein ZO-1
Alias SymbolsZO1, ZO-1
NCBI Gene Id403752
Protein NameTight junction protein ZO-1
Description of TargetThe N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.
Swissprot IdO97758
Protein Accession #NP_001003140.1
Nucleotide Accession #NM_001003140.1
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express TJP1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express TJP1.
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