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TJP1 ELISA Kit (Rat) (OKCA01006)

96 Wells
$600.00
In Stock
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Gene Symbol:
TJP1
Official Gene Full Name:
Tight junction protein ZO-1
NCBI Gene Id:
403752
Alias Symbols:
ZO1, ZO-1
Description of Target:
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.
Protein Name:
Tight junction protein ZO-1
Swissprot Id:
O97758
Protein Accession #:
NP_001003140.1
Nucleotide Accession #:
NM_001003140.1
Predicted Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity:
7.8 pg/mL
Kit Range:
31.2 pg/mL-2,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level TJP1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level TJP1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology TJP1 ELISA Kit (Rat) (OKCA01006) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for TJP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TJP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TJP1 captured in well.
Kit Component:
ComponentAmount
TJP1 Microplate96 Wells (12 x 8 Well strips)
TJP1 Lyophilized Standard2
100X Biotinylated TJP1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean n=4 (%)Range (%)
Neat9186-95
1:210297-107
1:49185-97
1:89791-103
Kit Recovery:
MatrixRecovery range (%)Average(%)
Serum (n=5)9589-98
EDTA Plasma (n=4)9790-100
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TJP1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TJP1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA

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