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Catalog No: OKEH08786
Size:96T
Price: $875.00
SKU
OKEH08786
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology TJP1 ELISA Kit (Dog) (OKEH08786) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TJP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TJP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TJP1 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=4.7% (n = 20)
Mean Inter-assay CV%: <=12.3% (n = 20)
ELISA Kit Component
ComponentAmount
TJP1 Microplate96 Wells (12 x 8 Well strips)
TJP1 Lyophilized Standard2
100X Biotinylated TJP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.
::Subcellular Location: Cell membrane Peripheral membrane protein Cytoplasmic side Cell junction tight junction Cell junction Cell junction gap junction Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells.
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.395ng/mL
SpecificityNatural and recombinant Dog Tight junction protein ZO-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTJP1
Gene Full Nametight junction protein 1
Alias SymbolsTight junction protein 1;tight junction protein 1 (zona occludens 1);tight junction protein ZO-1;ZO1;ZO-1;Zona occludens protein 1;zonula occludens protein 1.
NCBI Gene Id403752
Protein NameO97758
Description of TargetTight junction protein ZO-1
Uniprot IDhttps://www.uniprot.org/uniprot/O97758
Protein Accession #NP_001003140.1
Nucleotide Accession #NM_001003140.1
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