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TJP1 Antibody (OAGA00704)

100 ul
$409.00
In Stock
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Gene Symbol:
TJP1
Official Gene Full Name:
Tight junction protein ZO-1
NCBI Gene Id:
7082
Protein Name:
Tight junction protein ZO-1
Swissprot Id:
Q07157
Protein Accession #:
NP_003248.3
Alias Symbols:
DKFZp686M05161 antibody, MGC133289 antibody, ZO-1 antibody, TJP1 antibody, zona occludens protein 1 antibody, zona occludens 1 antibody, tight junction protein ZO-1 antibody, zonula occludens 1 protein antibody, zonula occludens protein 1 antibody, tight
Replacement Item:
This antibody may replace item sc-10804 from Santa Cruz Biotechnology.
Molecular Weight:
187 kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Purified by antigen-affinity chromatography.
Application:
ICC, IF, IHC-FFPE, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TJP1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TJP1.
Immunogen:
Recombinant fragment corresponding to a region within amino acids 1 and 296 of ZO-1 (Uniprot ID#Q07157)
Predicted Species Reactivity:
Human
Product Format:
Liquid
Reconstitution and Storage:
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Concentration:
1 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-TJP1 antibody - OAGA00704
Isotype:
IgG
Application Info:
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Additional Information:
Background: This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
:::
Positive Controls: H1299
:::
Cellular Localization: Cell membrane; Peripheral membrane protein; Cytoplasmic side, Cell junction, tight junction
Storage Buffer:
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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