Tissue factor/F3 ELISA Kit (Mouse) (OKBB00624)

Catalog No: OKBB00624

Size:96 Wells

Mouse Tissue factor/F3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse Tissue factor ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Tissue factor has been precoated onto 96-well plates. Standards(NS0, A29-E251) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tissue factor is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Tissue factor amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	113	327	603	120	315	592
  SD	4.41	14.4	30.8	5.64	18.3	37.3
  CV%	3.9	4.4	5.1	4.7	5.8	6.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse Tissue factor Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse Tissue factor Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse Tissue factor Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and Cell lysates
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse Tissue factor
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A29-E251
• Description: For quantitative detection of mouse Tissue factor in cell culture supernatants, serum, plasma(heparin, EDTA, citrate) and cell lysates.

Target Info

• Gene Symbol: F3
• Gene Full Name: coagulation factor III (thromboplastin, tissue factor)
• Alias Symbols: CD142, Coagulation factor III, F3, TF, TF_HUMAN, TFA, Thromboplastin, Tissue factor
• NCBI Gene Id: 14066
• Protein Name: Tissue factor
• Description of Target: Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It is mapped to 16q22.1. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 is a recently discovered cytokine that acts on tissue resident macrophages and Langerhans cells upon binding the receptor for CSF-1, CSF-1R. It also can promote the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor. In addition, it promotes the development, survival, and function of microglia and Langerhans cells, therefore, this cytokine may predominately function in brain and skin biology.
• Uniprot ID: P20352
• Protein Accession #: NP_034301.3
• Nucleotide Accession #: NM_010171.3