TINAGL1 ELISA Kit (Mouse) (OKEH07869)

Catalog No: OKEH07869

Size:96T

Mouse TINAGL1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	105-115% (n = 20)	107-117% (n = 20)	82-95% (n = 20)	107-117% (n = 20)
  EDTA plasma(n=5)	88-98% (n = 20)	96-108% (n = 20)	83-93% (n = 20)	96-104% (n = 20)
  heparin plasma(n=5)	93-102% (n = 20)	101-112% (n = 20)	110-119% (n = 20)	110-120% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology TINAGL1 ELISA Kit (Mouse) (OKEH07869) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TINAGL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TINAGL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TINAGL1 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.0% (n = 20)
  Mean Inter-assay CV%: <=8.4% (n = 20)
• ELISA Kit Component:
  • TINAGL1 Microplate: 96 Wells (12 x 8 Well strips)
  • TINAGL1 Lyophilized Standard: 2
  • 100X Biotinylated TINAGL1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: May be implicated in the adrenocortical zonation and in mechanisms for repressing the CYP11B1 gene expression in adrenocortical cells. This is a non catalytic peptidase C1 family protein.
  Subcellular Location: Secreted
  Unigene: Mm.15801
  String: 10090.ENSMUSP00000030560
  KEGG: mmu:94242
  Pfam: PF00112
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.2ng/mL
• Specificity: Natural and recombinant Mouse Tubulointerstitial nephritis antigen-like
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Tinagl1
• Gene Full Name: tubulointerstitial nephritis antigen-like 1
• Alias Symbols: 1110021J17Rik;adrenocortical zonation factor 1;androgen-regulated gene 1 protein;Ar;Arg1;AZ-;AZ1;AZ-1;Lc;Lcn7;TARP;Tinagl;tubulointerstitial nephritis antigen-like;tubulointerstitial nephritis antigen-related protein.
• NCBI Gene Id: 94242
• Protein Name: Q99JR5
• Description of Target: Tubulointerstitial nephritis antigen-like
• Uniprot ID: https://www.uniprot.org/uniprot/Q99JR5
• Protein Accession #: NP_001161805.1
• Nucleotide Accession #: NM_001168333.1